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KMID : 0613820110210040554
Journal of Life Science
2011 Volume.21 No. 4 p.554 ~ p.560
Isolation of Microorganisms and Development of Microbial Augmentation for Treatment of Paper Mill Wastewater
Kang Dae-Ook

Suh Hyun-hyo
Abstract
This study was performed to investigate the effects of microbial augmentation on the biological treatment of paper mill wastewater. Three bacteria (KN11, KN13, KN27) capable of degrading aromatic compounds and a bacterial strain (GT21) producing an extracellular cellulase were isolated from soil and wastewater by selective enrichment culture. Through morphological, physiological, and biochemical taxonomies, isolated strains of KN11, KN13, KN27, and GT21 were identified as Acinetobactersp., Neisseriasp., Bacillussp., and Pseudomonassp. and named Acinetobactersp. KN11, Neisseriasp. KN13, Bacillussp. KN27, and Pseudomonassp. GT21, respectively. For analysis of non-biodegradable and chemical oxygen demand (COD)-increasing matter in a paper mill wastewater, we utilized GC/MS to detect aromatic compounds and their derivatives containing several substituted functional groups. The microbial augmentation, J30 formulated with the mixture of bacteria including Acinetobactersp. KN11, Neisseriasp. KN13, Bacillussp. KN27, and Pseudomonassp. GT21, was used for the treatment of paper mill wastewater. The optimum temperature and pH for COD removal of the microbial augmentation, J30, were 30¡É and 7.5, respectively. For evaluation of the industrial applicability of the microbial augmentation, J30 in the pilot test, treatment efficiency was examined using paper mill wastewater. The microbial augmentation, J30, showed a COD removal rate of 87%. On the basis of the above results, we designed the wastewater treatment process of the activated sludge system.
KEYWORD
Mircobial augmentation, chemical oxygen demand, wastewater treatment, pilot test
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